Released on: January 22, 2008, 12:02 pm

Press Release Author: AnaSpec Inc.

Industry: Biotech

Press Release Summary: AnaSpec has introduced a new line of HABA Biotin Quantitation
Kits. They provide a convenient method for estimating the molar ratio of biotin to
protein on biotinylated conjugates and also be used to quantitate the biotin
concentration in a solution

Press Release Body: January 22, 2008 - San Jose, CA

AnaSpec has introduced a new line of HABA Biotin Quantitation Kits. These kits
provide a convenient method for estimating the molar ratio of biotin to protein on
biotinylated conjugates. They can also be used to quantitate the biotin
concentration in a solution.

Available in two sizes:
120 Assays 72096-120 (120 assays in a 96-well format or 25 assays in a cuvette format)
500 Assays 72096-500 (500 assays in a 96-well format or 100 assays in a cuvette format)

Biotin and avidin (or streptavidin) bind non-covalently with a higher binding
affinity than most antigen-antibody interactions. This very tight binding makes
labeling proteins with biotin a useful tool for applications such as affinity
chromatography and immunoanalytical assays.

The assay utilizes the observation that HABA (4'-hydroxyazobenzene-2-carboxylic
acid) shows dramatic spectral changes when it binds to avidin. Free HABA has an
absorption peak at 348 nm, while the HABA/avidin complex has strong absorption at
500 nm. Since the affinity between HABA and avidin is relatively weak (Kd=5.8 x 10-6
M) compared to the affinity between biotin and avidin (Kd=1 x 10-15 M), biotin can
easily replace HABA from the HABA/avidin complex, resulting in a decrease of
absorption at 500 nm. Avidin and HABA at an optimal ratio are included in the assay
kit. The kit has a linear range of 2 to 16 M.

Company Info

AnaSpec, Inc. is a leading provider of integrated proteomics solutions to
pharmaceutical, biotech, and academic research institutions throughout the world.
With a vision for innovation through synergy, AnaSpec focuses on three core
technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and
combinatorial chemistry.
For more information, visit www.anaspec.com



Web Site: http://www.anaspec.com

Contact Details: AnaSpec Inc.
2149 O\'Toole Ave.
San Jose, CA 9531
ping@anaspec.com